The Shimadzu SPD-20A operates on the principle of UV-Vis absorbance detection. The detector consists of a light source, a beam splitter, and a photodiode array. The light source emits a beam of light that passes through the flow cell, where the sample is detected. The beam splitter divides the light beam into two parts: one part passes through the sample, while the other part serves as a reference.
The SPD-20A is a standard UV-Vis absorbance detector, while its sibling model, the SPD-20AV, adds visible light capabilities via a halogen lamp. Understanding its core specifications helps maximize its performance in your assays. Key Specifications Deuterium ( D2cap D sub 2 ) lamp (plus a Halogen lamp for the SPD-20AV variant).
: Details technical adjustments for optical and electrical systems, including mirror and filter calibration. Technical Specification Sheets
190–700 nm (SPD-20A), 190–900 nm (SPD-20AV).
Flush with HPLC-grade water, followed by a mild 0.1M Nitric Acid ( HNO3cap H cap N cap O sub 3 shimadzu spd20a user manual
Switch off the unit, open the front panel, and carefully replace the lamp without touching the bulb with bare hands. B. Cleaning the Flow Cell
Ultimate Guide to the Shimadzu SPD-20A UV-Vis Detector: Operation, Maintenance, and Troubleshooting
Unscrew the protective lamp cover on the left-hand side of the unit.
The is more than just a document; it is an essential tool that ensures your investment in high-quality HPLC analysis is protected. From understanding the technical specifications to performing daily maintenance and troubleshooting complex optical errors, the official manual provides the authoritative guidance needed to keep your laboratory running smoothly. Always keep both the Instruction Manual and Service Manual (for qualified personnel) accessible. The Shimadzu SPD-20A operates on the principle of
Connect the capillary tubing from the column outlet to the flow cell inlet port (typically the bottom port).
Increase the flow rate slightly, or briefly restrict the waste line to increase cell backpressure (do not exceed 12 MPa).
Loosen the thumb screws on the right side panel of the detector and slide the cover off.
Comprehensive Guide to the Shimadzu SPD-20A Go to product viewer dialog for this item. User Manual: Operation, Maintenance, and Troubleshooting The Shimadzu SPD-20A Go to product viewer dialog for this item. The beam splitter divides the light beam into
Install a backpressure regulator on the waste line to prevent bubble formation inside the cell.
: Outlines the 190–700 nm wavelength range and noise levels ( SHIMADZU CORPORATION Quick Setup & Operation
The user manual provides detailed diagrams of the detector components. Key areas for operators to familiarize themselves with include:
Always ensure the waste line does not create excessive backpressure. The standard analytical flow cell has a maximum pressure tolerance of 12 MPa (approx. 120 bar). Exceeding this will rupture the cell quartz windows. 3. Daily Operation Guide